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. 2020 Jul 28;11:886. doi: 10.3389/fpls.2020.00886

TABLE 2.

The antimicrobial activity of phenolic acids against infection process of Phytophthora sojae according their concentrations in rhizosphere soil of maize.

Concentration Compound Chemotaxis Inhibition ratio (%)
Zoospore motility Cystospore germination Hyphal growth
× 0.1 (0.1 times) p-Coumaric acid 0.40 ± 0.12a 2.33 ± 1.20c 12.67 ± 0.33bc 37.33 ± 0.88a
p-Hydroxybenzoic acid 0.53 ± 0.07a 4.67 ± 0.88b 8.33 ± 2.73c 9.00 ± 4.36b
Vanillic acid 0.27 ± 0.07a 0.00 ± 0.00d 7.33 ± 2.73c 49.33 ± 6.36a
Ferulic acid 0.20 ± 0.18a 0.00 ± 0.00d 7.33 ± 0.88c 37.00 ± 10.54a
Cinnamic acid 0.13 ± 0.07a 4.00 ± 0.33bc 23.33 ± 4.05a 32.33 ± 13.20ab
mixture 0.20 ± 0.12a 10.33 ± 0.88a 21.00 ± 4.00ab 32.33 ± 3.92ab
× 0.5 (0.5 times) p-Coumaric acid 0.53 ± 0.07a 8.33 ± 1.76b 10.33 ± 3.18b 49.67 ± 1.45a
p-Hydroxybenzoic acid 0.33 ± 0.07ab 8.33 ± 2.96b 10.00 ± 4.00b 23.33 ± 7.36b
Vanillic acid 0.13 ± 0.07b 4.33 ± 1.45b 10.67 ± 2.19b 50.00 ± 3.61a
Ferulic acid 0.47 ± 0.07a 4.67 ± 2.73b 8.67 ± 1.45b 25.33 ± 4.67b
Cinnamic acid 0.33 ± 0.07ab 6.67 ± 2.33b 37.33 ± 7.84a 36.33 ± 9.87ab
mixture 0.07 ± 0.07b 44.67 ± 0.88a 27.33 ± 11.67ab 43.67 ± 7.31ab
× 1 (1 times) p-Coumaric acid 0.20 ± 0.07b 17.00 ± 4.73b 13.67 ± 2.73d 69.33 ± 15.41a
p-Hydroxybenzoic acid 0.20 ± 0.12b 7.00 ± 2.08c 13.33 ± 2.03d 24.33 ± 8.09c
Vanillic acid 0.07 ± 0.07b 6.67 ± 2.85c 23.67 ± 3.76c 33.67 ± 9.39bc
Ferulic acid 0.07 ± 0.07b 6.67 ± 0.33c 27.67 ± 4.91bc 37.33 ± 4.91bc
Cinnamic acid 0.97 ± 0.16a 15.67 ± 0.88b 37.33 ± 1.45b 25.67 ± 4.41bc
mixture 0.90 ± 0.10a 60.00 ± 2.52a 50.00 ± 4.51a 52.67 ± 1.67ab
× 5 (5 times) p-Coumaric acid 0.20 ± 0.07c 22.33 ± 1.76b 14.00 ± 1.53d 80.00 ± 2.52a
p-Hydroxybenzoic acid 0.73 ± 0.24b 10.00 ± 2.31c 20.00 ± 2.89cd 29.67 ± 2.40c
Vanillic acid 0.07 ± 0.07c 11.33 ± 1.20c 21.00 ± 3.46cd 40.33 ± 3.48c
Ferulic acid 0.60 ± 0.07b 10.67 ± 0.88c 29.33 ± 3.28c 45.00 ± 16.52bc
Cinnamic acid 1.27 ± 0.02a 18.67 ± 1.45b 43.67 ± 6.36b 37.00 ± 3.51c
mixture 1.45 ± 0.02a 87.67 ± 0.67a 60.67 ± 2.40a 66.33 ± 7.88ab
× 10 (10 times) p-Coumaric acid 0.40 ± 0.07c 29.33 ± 4.37c 57.33 ± 1.45b 90.67 ± 2.03a
p-Hydroxybenzoic acid 0.53 ± 0.13c 21.33 ± 1.86cd 10.00 ± 1.00d 41.33 ± 7.31b
Vanillic acid 0.13 ± 0.13c 19.67 ± 2.40d 18.67 ± 4.41c 66.00 ± 7.23a
Ferulic acid 0.20 ± 0.13c 22.33 ± 3.18cd 56.33 ± 1.67b 31.00 ± 10.82b
Cinnamic acid 1.30 ± 0.12b 38.00 ± 1.73b 58.00 ± 1.15b 41.00 ± 11.01b
mixture 1.68 ± 0.13a 100.00 ± 0.00a 68.00 ± 1.73a 87.00 ± 2.52a

Mixture is consisted of five phenolic acid compounds according to their concentrations and proportion in the rhizosphere soil of maize. The concentration with (× 0.1), (× 0.5), (× 1), (× 5) and (× 10) indicate that the concentrations of mixture were 0.1, 0.5, 1.0, 5.0 and 10.0 times of the rhizosphere soil concentration (Mixture = 0.15 mg/L cinnamic acid + 26.13 mg/L p-coumaric acid + 4.93 mg/L vanillic acid + 0.50 mg/L ferulic acid + 17.11 mg/L p-hydroxybenzoic acid). Different letters in the same group indicate significant differences by ANOVA (P < 0.05). The error indicates standard error of means (n = 3).