Figure 4.

The cell proliferation rate of different cell lines compared to the control and shFRAT2 groups. (A) Five breast cancer cell lines were used to detect the expression level of FRAT2. Among these 5 cell lines, the T47D cells had the highest expression levels, while MDA-MB-231 cells had the lowest expression levels. These two cell lines are also BLBC cell lines. Subsequently, shMONK, shFRAT2 and shRNA targeted against FOXM1 were transfected into T47D and MDA-MB-231 cells, and these transfected cells were named the shCtrl, shFRAT2 and shPC group, respectively. The shCtrl group cells were transfected with MONK, which has no effect on cell proliferation; therefore, the T47D and MDA-MB-231 cells could divide normally. (B) We constructed 4 plasmids, including a control and 3 shFRAT2 plasmids. shFRAT2-1 showed the most significantly silencing effect. (C) Three constructed shRNAs were also detected by Western blotting assay. FRAT2 silencing effects were all significant in these three plasmids. shFRAT2-1 was the most significant. FRAT2, frequently rearranged in advanced T-cell lymphomas 2.