Figure 1.

If tissue samples (histological or cytological) were inadequate or insufficient at morphological evaluation for molecular analysis (A, B), liquid biopsy may represent a useful tool (C). In our study, after blood withdrawn (C), plasma was obtained after centrifugation (D) and extracted ctDNA was further analyzed by using our next generation sequencing panel (SiRe^®) on S5 platform (Thermo Fisher Scientific, Waltham, MA) (E). Visual inspection of the sequencing reads was performed by using the Golden Helix Genome Browser v.2.0.7 (Bozeman, MT, USA) (F). (Credit: Created with Biorender).