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. 2020 Jul 24;12:6279–6289. doi: 10.2147/CMAR.S253327

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© 2020 Huang and Du.

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Combination effect of PUE and DDP on inducing apoptosis and anti-proliferation of A549/DDP cells compared with the monotherapy of PUE or DDP. (A) Determination of the cytotoxicity of PUE, DDP, and PUE + DDP to A549 cells and A549/DDP cells, respectively, by cell apoptosis experiments. (B) Anti-proliferation effect of PUE, DDP, and PUE + DDP on A549 cells and A549/DDP cells evaluated by the cell cloning assay. Qualitative (C) and quantitative (D) analysis of the effect of PUE, DDP, and PUE + DDP on the regulation of cell proliferation-related genes expressions in A549/DDP cells by Western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.05, ^###p < 0.05, compared with controls.