Figure 1.
Cell width and MreB localization are disrupted by CC_2490 overexpression. (A) CC_2490 expression was induced in wild-type C. crescentus for the indicated times. Phase-contrast images show disruption to cell width and cell shape in C. crescentus cells overexpressing CC_2490. Scale bars, 2 μm. (B) MreB-GFPsw cells with or without CC_2490 overexpression (Pxyl-cc_2490-FLAG) were imaged by phase and fluorescence microscopy at 9 h postinduction. MreB was delocalized in cells grown with CC_2490 overexpression. Arrows indicate examples of MreB localization. Scale bars, 2 μm. (C) Expression of CC_2490 was determined by Western blot using FLAG-tagged cc_2490 expressed from its native locus. Overexpression of CC_2490-FLAG was induced with 0.3% xylose for 9 h. Image quantification shows that xylose induction resulted in 1.7-fold overexpression. To see this figure in color, go online.