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ITC of the association of PDZ1, PDZ2, and PDZ1-2 with the RRESEI ligand. ITC traces (top) and saturation analysis (bottom) for PDZ1, PDZ2, and PDZ1-2, each titrated with the RRESEI ligand, are shown overlaid. The heat change on successive injections of ligand is shown in the top panel buffer heat of dilution subtraction (baseline in red). The corresponding binding isotherm with fitted curve is shown in the bottom panel. To see this figure in color, go online.
