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. 2020 Jul 28;10:375. doi: 10.3389/fcimb.2020.00375

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Copyright © 2020 Hsieh, Tsai, Chen, Shen, Jhan, Tseng and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased expression of DC-SIGN in D-galactose-treated THP-1 cells. (A) Immunostaining followed by flow cytometric analysis showing the expression of DC-SIGN in the D-gal-stimulated THP-1 cells 48 h posttreatment. A representative histogram and the percentage of positive cells are shown, and the data are the mean ± SD of three independent experiments. (B) Flow cytometry measured the infectivity of fluorescent Alexa-594-labeled DENV2 (at a multiplicity of infection of 1) 2 h post-infection in D-galactose (D-gal; 250 mM)-treated THP-1 cells in the presence and absence of neutralizing anti-DC-SIGN and control IgG (5 μg/ml). **p < 0.01 compared to the DENV-infected cells. ^#p < 0.05, compared to the DENV-infected senescent cells.