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. 2020 Jul 9;20(3):2928–2936. doi: 10.3892/ol.2020.11839

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Copyright: © Niu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — The expression of EMT-associated proteins and MMPs was examined by western blotting after (A) T24 and (B) 5637 cells were treated with different concentrations of PPII. ACTB was used as a loading control. The relative expression of proteins in (C) T24 and (D) 5637 cells treated with PPII at different concentrations was calculated. Experiments were conducted in triplicate. NS, not significant; *P<0.05; **P<0.01; ***P<0.001. PPII, polyphyllin II; ACTB, β-actin; CDH1, E-cadherin; CDH2, N-cadherin; SNAI2, snail family transcriptional repressor 2; TWIST1, twist family bHLH transcription factor 1; MMP, matrix metallopeptidase.