Figure 1.

In vivo effects of Thykamine in animal models of acute inflammation. Hapten 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis in rats was measured by (A) the macrocsopic damage and (B) the ratio weight/length. Male Wistar rats, after an overnight food deprivation, were anesthetized with isoflurane before insertion of a colonic catheter of 8 cm. The TNBS (25 mg/mL in 50% aqueous/ethanol; vol/vol) was injected into rat colon (total volume injected: 1 mL/rat). The control rats (n = 4) received 1 mL of vehicle (aqueous/ethanol; vol/vol) intracolonically. Rats were injected intraperitoneally with Thykamine (5 mg/kg, n = 4) in sterile physiologic saline (1 mL) immediately prior to anesthesia. Statistics: Paired 2-tailed t test (significance for p value < 0.05). (C) Carrageenan-induced paw edema in rats. Male Wistar rats pre-fasted overnight received Thykamine (0, 0.5, 5 or 50 mg/kg; n = 9, 5, 6, 5, respectively) in sterile physiologic saline by intraperitoneal injection (1 mL) immediately prior to subplantar injection of carrageenan (0.1 mL of 1% suspension in 0.9% saline) in the right hind paw. Paw circumference was measured immediately prior to carrageenan injection and 5 h afterwards. Edema was expressed in mm as the increase of paw circumference measured after carrageenan injection and compared to the pre-injection value of individual animals. Statistics: paired 2-tailed t test (significance for p value < 0.05).