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. 2020 Jun 28;9(7):520. doi: 10.3390/pathogens9070520

Figure 4.

Figure 4

Regulation of H3 methylation by bPRL and E2 in bMECs during infection. Densitometrical analysis of the immunoblots that shows the relative expression of H3K9me2 with respect to total H3 in bMECs treated for 12 h (a) or 24 h (b) with the combined hormones. In addition, representative western blot analysis is shown. Each bar shows the mean ± SE of optical density (arbitrary units, AU), considering the expression of control cells (1% ethanol) as 1 (data normalized), from four different experiments (n = 4). (c) bMEC lysates were obtained at 24 h of treatment with combined hormones with or without 2 h of S. aureus infection, and were incubated with the substrates for Jumonji demethylases (HDMs). Each bar shows the mean of the HDM activity of cell lysates from two different experiments ± SE, which were run in duplicate (n = 4), considering the expression of control cells (1% ethanol) as 1 (data normalized). Different letters indicate significant differences between each condition (one-way ANOVA, post hoc Tukey test p ≤ 0.05. Veh: vehicle; Sa: S. aureus; H: combined hormones.