Table 4.
Oligonucleotides of inflammatory response and epigenetic genes used in this study.
| Genes (bovine) 1 | Primer | Sequence (5′→3′) | Fragment (bp) |
Tm (°C) |
Reference |
|---|---|---|---|---|---|
| BNBD10 | F R |
GCTCCATCACCTGCTCCTC AGGTGCCAATCTGTCTCATGA |
152 | 54 | [15] |
| DEFB1 | F R |
CCATCACCTGCTCCTCACA ACCTCCACCTGCAGCATT |
185 | 54 | [15] |
| GAPDH | F R |
TCAACGGGAAGCTCACTGG CCCCAGCATCGAAGGTAGA |
237 | 57 | [15] |
| HDAC1 | F R |
TTACGACGGGGATGTTGGAA GGCTTTGTGAGGACCGTTGG |
136 | 62 | This work |
| IL-10 | F R |
GATGCGAGCACCCTGTCTGA GCTGTGCAGTTGGTCCTTCATT |
129 | 59 | [12] |
| IL-1α | F R |
GCAGAAGGGAAGGGAAGAATGTAG CAGGCTGGCTTTGAGTGAGTAGAA |
198 | 52 | [15] |
| IL-6 | F R |
AACCACTCCAGCCACAAACACT GAATGCCCAGGAACTACCACAA |
179 | 57 | [12] |
| CXCL8 | F R |
TTCCACACCTTTCCACCCCAA GCACAACCTTCTGCACCCACTT |
149 | 53.5 | [12] |
| KDM4A | F R |
CAGAAATGTGCTTCACCTCG CACCGAGAACACATCCAGTC |
178 | 58 | This work |
| KDM4C | F R |
GGAACACCCGGTACTACAG CACTTGACTTGCACGACTTC |
154 | 62 | This work |
| LAP | F R |
GCCAGCATGAGGCTCCATC CTCCTGCAGCATTTTACTTGGGCT |
194 | 54 | [15] |
| TAP | F R |
GCGCTCCTCTTCCTGGTCCTG GCACGTTCTGACTGGGCATTGA |
216 | 57 | [15] |
| TNF-α | F R |
CCCCTGGAGATAACCTCCCA CAGACGGGAGACAGGAGAGC |
101 | 56 | [12] |
1 BNDB10: Bovine neutrophil beta defensin 10; DEFB1: Defensin beta 1; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; HDAC1: Histone deacetylase 1; IL-10: Interleukin-10; IL-1β: Interleukin 1-β; IL-6: Interleukin-6; CXCL8: Chemokine interleukin-8; KDM4A: Lysine demethylase 4A; KDM4C: Lysine demethylase 4C; LAP: Lingual antimicrobial peptide; TAP: Tracheal antimicrobial peptide; TNF-α: Tumor necrosis factor α.