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. 2020 Jul 15;17(14):5120. doi: 10.3390/ijerph17145120

Table 3.

Primers and probes used in digital droplet PCR [20,35,36].

Target Primers and Probe Sequences 5′-3′ Reference
F. prausnitzii Fpra_428_F TGTAAACTCCTGTTGTTGAGGAAGATAA [20]
Fpra_583_R GCGCTCCCTTTACACCCA
Fpra_493_PR FAM/CAAGGAAGTGACGGCTAACTACGTGCCAG/IABkFQ
E. coli Ecoli_395_F CATGCCGCGTGTATGAAGAA [35]
Ecoli_490_R CGGGTAACGTCAATGAGCAAA
Ecoli_437_PR FAM/TATTAACTTTACTCCCTTCCTCCCCGCTGAA/IABkFQ
C. coccoides F_Ccoc_07 GACGCCGCGTGAAGGA [36]
R_Ccoc_14 AGCCCCAGCCTTTCACAT
P_Erec_482 VIC/CGGTACCTGACTAAGAAG/IABkFQ
Bacteria F_Bact_1369 CGGTGAATACGTTCCCGG [36]
R_Prok_1492 TACGGCTACCTTGTTACGACTT
P_TM_1389F FAM/CTTGTACACACCGCCCGTC/IABkFQ