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Photograph of agarose gel for samples of NUCL patients. (A): genus-specific PCR revealing a 120 bp fragment of Leishmania kDNA. (B): PCR-RFLP after Hae III digestion of a 234 bp Hsp70 fragment. Columns 1–3: lesion scraping samples from NUCL patients; columns 4-6: Leishmania (L.) infantum (MHOM/BR/1972/BH46); Leishmania (L.) amazonensis (MHOM/BR/1973/M2269), Leishmania (V.) braziliensis (MHOM/BR/1995/M15280), respectively. NC: negative control; MW: ladder.
