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Resolution of plasmid dimers. Dimers of the plasmid pKD3 were introduced by transformation into E. coli DS981XerCAb or E. coli DS99028XerDAb. The cells were cultured overnight in a low or high osmolarity medium in the presence of 100 μg/mL of ampicillin. Plasmid DNA was isolated and subjected to agarose gel electrophoresis. The bands below the dimer correspond to the complementing plasmid. The position of migration of the dimers and monomers are indicated to the sides.
