Table 2.
Bacterial strains and plasmids used in this study.
| Bacterial Strain or Plasmid | Relevant Characteristics, Genotype, or Phenotype a | Source or Reference |
|---|---|---|
| E. coli strains | ||
| DS941 | AB1157 recF143 lacIq lacZΔM15 | [65] |
| DS981 | DS941 xerC (Kanr) | [56] |
| DS9028 | DS941 xerD (Tmpr) | [57] |
| DS981XerCAb | DS981 (pMSR1) (Kanr Tetr) | This work |
| DS9028XerDAb | DS9028 (pMSR2) (Tmpr Tetr) | This work |
| DS9040 | DS941 xerC xerD (Kanr Genr) | [34] |
| JC8679 | DS945 recBC sbcA (hyperrecombinogenic) | [39] |
| A. baumannii strain | ||
| A118 | Human clinical isolate | [58] |
| Plasmids | ||
| pMSR1 | xerCAb cloned into the pACYC184 EcoRI site (Tetr) | This work |
| pMSR2 | xerDAb cloned into the pACYC184 EcoRI site (Tetr) | This work |
| pBAD102xerCAb | xerCAb cloned into pBAD102 (Ampr) | This work |
| pBAD102xerDAb | xerDAb cloned into pBAD102 (Ampr) | This work |
| pKD3 | EcoRI-SacI fragment containing the pJHCMW1 mwr site with substitution C to T at the ArgR binding site cloned in pUC18 (Ampr) | [8] |
| pUC18 | Cloning vector (Ampr) | [60] |
| pCR2.1 | Cloning vector (Ampr, Kanr) | ThermoFisher |
| pACYC184 | Cloning vector, p15A replicon (Chlr Tetr) | [61] |
a Amp, ampicillin; Chl, chloramphenicol; Gen, gentamicin; Kan, kanamycin; Tet, tetracycline; Tmp, trimethoprim.