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. 2020 Jul 1;20(3):2191–2198. doi: 10.3892/ol.2020.11794

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Establishment of METTL3-KO AGS cells. AGS cells were transfected with METTL3 CRISPR/Cas9 and homology-directed repair plasmids. Colonies were selected and confirmed by detecting METTL3 expression and global m6A levels. (A) Stable METTL3-KO AGS cells (AGS-C20 and AGS-C43) were selected and METTL3-KO was confirmed via PCR analysis of METTL3 RNA. (B) Stable METTL3-KO AGS cells (AGS-C20 and AGS-C43) exhibited a significant decrease or a truncated expression of METTL3 protein, as determined via western blotting. Representative images are presented. Densitometric levels of positive bands were quantified and are expressed as the ratio to β-actin. (C) A significant decrease in global m6A levels was detected in METTL3-KO AGS cells. *P<0.05 vs. AGS-WT. METTL3-KO, methyltransferase like 3 knocked down; WT, wild type.