Table 2.

Nucleotide and amino acid alterations and mRNA expression changes among five pairs of isogenic Klebsiella pneumoniae strains.

Set	Gene Name	Function	Nucleotide Alteration	Amino Acid Alteration	Fold Change of mRNA Expression
B0608-134 versus B0608-134R	Intergenic region between KPN_02065 and KPN_02066	KPN_02065, putative transport protein KPN_02066, putative response regulator consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain	Insertion of Insertion sequence (IS)5-like element	-	208.99 ± 48.27-fold increase for KPN_02065
					4.48 ± 0.1-fold increase for KPN_02066
B0704-039 versus B0704-039R1	Intergenic region between KP1_3468 and KP1_3469	KP1_3468; conserved hypothetical protein KP1_3469; putative outer membrane	Insertion of IS5-like element	-	2.51 ± 0.49-fold increase for KP1_3468
					1.03 ± 0.11 fold increase for KP1_3469
B0704-039 versus B0704-039R2	KP1_3468	Hypothetical protein	T26A, C108G, A128T	L9Q, S36R, K43I	7.91 ± 0.31-fold increase
	mgrB	PhoPQ regulatory protein	Insertion of IS5-like element	Frameshift	-
B08-063 versus B08-063R	phoQ	Sensor protein PhoQ	A803C	Y268S	7.49 ± 0.52-fold increase
			12-bp deletion at 341st nucleotide	Deletion of 5 amino acids at position 114
	KP1_3620	Hypothetical protein	1 bp deletion at 51st nucleotide	Premature stop at position 14	-
	KP1_0061	Repressor of galETK operon	T104G	V35G	2.11 ± 0.35-fold increase
B0701-068 versus B0701-068R	KP1_3468	Hypothetical protein	Insertion of IS5-like element	Frameshift	0.9 ± 0.14-fold increase
	KPN_02067	Sensor histidine protein kinase (RstA regulator)	G593T	G198V	1.36 ± 0.05-fold increase
	mgrB	PhoPQ regulatory protein	Insertion of IS5-like element	Frameshift	-