Table 4.
Primers used in this study.
| Primer | Sequence (5′→ 3′) | Amplicon Size (bp) |
Reference |
|---|---|---|---|
| Primers for sequencing | |||
| 134-target1-F | TGAATGCACAAGGTAAAGCCAGG | 500 | This study |
| 134-target1-R | CCGTCTTCGGCTCTTAAGGTTTT | ||
| 039-target1-F | GACACAGCCAGCGATGCCAG | 416 | This study |
| 039-target1-R | TTGTATGATCCATGGCGTGA | ||
| 063-target1,2-F | ATGCAGGTAATAACCAATCT | 751 | This study |
| 063-target1,2-R | ATCCCGGAAAACCTGAATAT | ||
| 063-target3-F | CCATAATAAAAAAAATAATC | 362 | This study |
| 063-target3-R | GCCCCTCCCCACACATCTTT | ||
| 063-target4-F | ACGGCCGAGCGCCAGAAACG | 720 | This study |
| 063-target4-R | TCGCATCCGCATCCGCCAGG | ||
| 068-target1-F | TATGATGCACACCTGTCGGG | 780 | This study |
| 068-target1-R | CACTGTGGAATAACACCCCA | ||
| 068-target2-F | GGCACTGAATAATGCGTAAG | 480 | This study |
| 068-target2-R | GCCTGAAACAAATCTCTCAG | ||
| mgrB-ext-F | CAGCCAGCGATGCCAGATTT | 380 | [5] |
| mgrB-ext-R | CCTGGCGTGATTTTGACACGA | ||
| Primers for qRT-PCR | |||
| RT-KPN_02065-F | CCCCGGGGTGCTATCTCAC | 135 | This study |
| RT-KPN_02065-R | CCGCCCAGGATGGCATTTAT | ||
| RT-KPN_02066-F | TCGCTGCCATACTGACAGGCT | 148 | This study |
| RT-KPN_02066-R | TTCCAGCCATCGTACACGGG | ||
| RT-KP1_3468-F | AAAAATTACGGTGGGTTTTACTGA | 109 | This study |
| RT-KP1_3468-R | ATGCCGCTGAAAAACTGAAC | ||
| RT-KP1_3469-F | TGGATTGGCGTATTATTGAGC | 122 | This study |
| RT-KP1_3469-R | TGAACATAAAGTGCGGTGCT | ||
| RT-phoQ-F | TGCCAGGGAAGCGGACTAC | 100 | This study |
| RT-phoQ-R | GCGGCGGATCAGTGATAAAC | ||
| RT-KP1_0061-F | TTATCACCGACTGCAACACC | 138 | This study |
| RT-KP1_0061-R | CGACATCGGTCTCAATGATG | ||
| RT-KPN-02067-F | GGGAAACCTTAGTGCCAGAGC | 111 | This study |
| RT-KPN-02067-R | TGCGCATCCAGCGTCTGTAA | ||
| RT-rpoB-F | CGCGTATGTCCGATCGAAA | 100 | This study |
| RT-rpoB-F | GCGTCTCAAGGAAGCCATATTC | ||