. 2020 Jul 14;17(14):5048. doi: 10.3390/ijerph17145048

Table 1.

Primers and PCR cycling parameters for the detection of various potential bacterial pathogens and Candida albicans.

Organism	Primer Name	Primer Sequence (5′–3′)	PCR Cycling Parameters	Gene (Size [bp])	References
Universal	Forward: fDD2	CCGGATCCGTCGACAGAGTTTGATCITGGCTCAG	3 min at 94 °C; 30 cycles of 94 °C for 30 s, 53 °C for 30 s, 72 °C for 1.5 min	16S rRNA (1600)	[25,26]
Universal	Reverse: rPP2	CCAAGCTTCTAGACGGITACCTTGTTACGACTT		16S rRNA (1600)	[25,26]
Shigella spp.	Forward: IpaH-F	CCTTGACCGCCTTTCCGATA	2 min at 95 °C; 35 cycles of 94 °C for 1 min, 62 °C for 1 min, 72 °C for 2.5 min, 72 °C for 3 min	Invasion plasmid antigen H (606)	[25,26]
Shigella spp.	Reverse: IpaH-R	CAGCCACCCTCTGAGGTACT		Invasion plasmid antigen H (606)	[25,26]
Legionella spp.	Forward: JFP	AGGGTTGATAGGTTAAGAGC	5 min at 95 °C; 40 cycles of 94 °C for 1 min, 57 °C for 1.5 min, 72 °C for 1 min, 72 °C for 5 min.	Attachment invasion locus gene (386)	[25,26]
Legionella spp.	Reverse: JRP	CCAACAGCTAGTTGACATCG		Attachment invasion locus gene (386)	[25,26]
Salmonella spp.	Forward: IpaB-F	GGACTTTTTAAAAGCGGCGG	2 min at 95 °C; 35 cycles of 94 °C for 1 min, 62 °C for 1 min, 72 °C for 2.5 min, 72 °C for 5 min.	Invasion plasmid antigen B (314)	[25,26]
Salmonella spp.	Reverse: IpaB-R	GCCTCTCCCAGAGCCGTCTGG		Invasion plasmid antigen B (314)	[25,26]
Pseudomonas spp.	Forward: PA-GS-F	GACGGGTGAGTAATGCCTA	2 min at 95 °C; 35 cycles of 94 °C for 20 s, 54 °C for 20 s, 72 °C for 40 s, 72 °C for 5 min	16S rRNA (618)	[25,26]
Pseudomonas spp.	Reverse: PA-GS-R	CACTGGTGTTCCTTCCTATA		16S rRNA (618)	[25,26]
Candida albicans	Forward: CALB1	TTTATCAACTTGTCACACCAGA	5 min at 95 °C; 35 cycles of 94 °C for 30 s, 58 °C for 30 s, 72 °C for 30 s, 72 °C for 10 min.	ITS-1, ITS-2 (278)	[34]
Candida albicans	Reverse: CALB2	ATCCCGCCTTACCACTACCG		ITS-1, ITS-2 (278)	[34]