Table 3.

Primers and PCR cycling parameters for the detection of E. coli pathotypes according to Ramírez Castillo and colleagues [8].

Organism.	Primer Name	Primer Sequence (5′–3′)	PCR Cycling Parameters	Gene (Size [bp])
EAEC	Forward: AggRKs1	GTATACACAAAAGAAGGAAGC	Stage 1, initial denaturing at 95 °C for 2 min; stage 2, denaturing at 95 °C for 1 min, primer annealing at 54 °C for 1 min, and elongation at 72 °C for 1 min; for 30 cycles, and stage 3, final elongation step at 72 °C for 10 min.	aggR (254)
	Reverse: AggRkas2	ACAGAATCGTCAGCATCAGC
EHEC	Forward: VTcomU	GAGCGAAATAATTTATATGTG		stx (518)
	Reverse: VTcomd	TGATGATGGCAATTCAGTAT
EPEC	Forward: SK1	CCCGAATTCGGCACAAGCATAAGC		eae (881)
	Reverse: SK2	CCCGGATCCGTCTCGCCAGTATTCG
EIEC	Forward: IpaIII	GTTCCTTGACCGCCTTTCCGATACCGTC		ipaH (619)
	Reverse: IpaIV	GCCGGTCAGCCACCCTCTGAGAGTAC