Table 2.

Physicochemical properties of different AMP-NPs at different peptide/polymer ratios.

NP	(µg AMP /mgPLGA) Ratio	Mean Zeta Potential (mV)			Mean Size (nm)			Mean PDI			Mean Encapsulation Efficiency (%)			Mean Peptide Loading (µg AMP/mg PLGA)
Empty NP	0	−21.97	±	1.25	270.87	±	2.96	0.11	±	0.03	-		-	-		-
G17NP	16	8.56	±	0.30	293.20	±	1.61	0.24	±	0.03	49.50	±	0.70	7.92	±	0.11
	8	7.26	±	1.11	284.50	±	3.61	0.22	±	0.02	90.59	±	0.67	7.25	±	0.05
	4	3.99	±	0.23	303.33	±	18.65	0.27	±	0.07	76.10	±	1.87	3.04	±	0.07
	2	−2.06	±	0.21	1022.40	±	317.18	0.83	±	0.27	67.58	±	0.83	1.35	±	0.02
	1	−14.10	±	0.56	322.27	±	4.25	0.22	±	0.02	83.79	±	0.14	0.84	±	0.00
	0.50	−19.03	±	0.64	339.53	±	1.86	0.12	±	0.08	86.19	±	3.59	0.43	±	0.02
	0.25	−20.80	±	0.46	285.37	±	1.61	0.08	±	0.04	87.64	±	3.66	0.22	±	0.01
	0.12	−23.37	±	0.45	266.30	±	4.35	0.12	±	0.04	88.48	±	3.01	0.11	±	0.00
G19NP	16	12.90	±	0.60	291.67	±	3.38	0.16	±	0.11	50.85	±	1.20	8.14	±	0.19
	8	12.87	±	1.02	291.77	±	1.59	0.26	±	0.06	90.60	±	0.94	7.25	±	0.07
	4	12.90	±	0.80	257.83	±	2.84	0.13	±	0.05	80.48	±	1.76	3.22	±	0.07
	2	3.03	±	0.30	290.70	±	3.67	0.08	±	0.04	79.27	±	2.26	1.59	±	0.04
	1	−1.86	±	0.37	432.63	±	6.66	0.16	±	0.04	51.10	±	4.98	0.51	±	0.05
	0.50	−11.80	±	0.95	1976.00	±	496.56	0.82	±	0.12	41.05	±	3.18	0.21	±	0.02
	0.25	−15.77	±	0.40	353.67	±	5.93	0.17	±	0.02	89.92	±	6.88	0.22	±	0.02
	0.12	−18.73	±	0.40	324.87	±	7.27	0.12	±	0.05	89.45	±	4.39	0.11	±	0.01

AMP-NP: Encapsulated Antimicrobial peptide; AMP: Antimicrobial peptide; PLGA: Poly-lactic-glycolic-acid; PDI: poly dispersion index.