Figure 4.

Positive and negative selection in the top ten most variable orthotospoviruses. (A) Frequency of the sites under positive selection normalized to the length of the cistron compared to the expected randomly (sites per cistron / total for the open reading frame). * denotes significant differences with p-value ≤ 0.001 as calculated by the Chi-square test. (B) Relative number of sites under positive selection for each species, expressed cumulatively by genomic RNA segment, and normalized to the length of the open reading frame. (C) Relative number of sites under negative selection.