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. 2020 Jul 9;20(3):2721–2728. doi: 10.3892/ol.2020.11837

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Notch2 is a direct target gene of miR-34b. (A) Predicted miR-34b binding sites in the 3′ UTR of Notch2 mRNA. (B) Luciferase activity in cells 48 h after transfection. (C) Relative expression levels of Notch2 in SKOV3 cells transfected with NC or miR-34b mimics. (D) and (E) Western blotting of Notch2 in SKOV3 cells transfected with NC or miR-34b mimics. **P<0.01 vs. NC. miR, microRNA; UTR, untranslated region; NC, negative control; ns, not significant.