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. 2020 Jul 19;9(7):589. doi: 10.3390/pathogens9070589

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Maximum-likelihood phylogenetic tree of estimating the relationships of selected West Nile virus isolates. The tree was constructed with MEGA-X software version 10.1.8. The optimal tree was obtained using Maximum likelihood method, Nearest Neighbour Interchange (NNI) inference method. The phylogeny was tested with bootstrap replicates method (N = 1000). The evolutionally distances were calculated with the general time reversible (GTR) model, uniform rates. The tree was edited with FigTree v1.4.3 software (http://tree.bio.ed.ac.uk/software/figtree/). The scale represents at the bottom represent divergence time in millions of years ago (MYA). Each sequence used was labelled by GenBank accession number_isolate/strain name_country of isolation_year of isolation.