Figure 2.

Biofilm inhibition by Cm-p5 derivatives. (a) Dose-dependent inhibition of C. auris de novo biofilm formation by cyclic Cm-p5, dimer 1 and dimer 2 quantified with crystal violet after 24 h. Inhibitors were present throughout the growth. R² represents the coefficient of determination of the applied trendlines. (b) Arrest of biofilm growth by Cm-p5 derivatives added to mature biofilms (at t = 0 and 24 h). (c) Inhibition calculated from (b) relative to the mature biofilm (t = 0 h). A constant biofilm biomass thereby depicts an inhibition of 100%. All experiments were performed in triplicate, and statistical analysis by a t-test was performed where applicable. p values < 0.05 were considered significant. * denotes p < 0.05, ** < 0.01, *** < 0.001.