Figure 1.

Effect of overexpression nc886 on the production of inflammation-related proteins increased by UVB. Cells were transfected with 1 µg of nc886 gene. Then, 48 h after transfection, the cells were irradiated with UVB (5 mJ/cm²). RNA samples were analyzed with quantitative real-time polymerase chain reaction (qRT-PCR) and the cell culture fluid with ELISA. Values measured for (A) nc886, (B) MMP-9, (C) COX-2 were indicated in a fold change compared to the control. The mRNA level was normalized with the expression of 18S rRNA. The measured values for (D) MMP-9, (E) PGE₂, (F) IL-8, and (G) TNF-α were indicated in absolute concentration for each. * p < 0.05, # p < 0.01, compared to basal levels; ^o p < 0.05, ^$ p < 0.01, compared between (−) UVB and (+) UVB groups.