Figure 2.
Analysis of the expression and methylation changes of nc886 in human keratinocytes exposed to ultraviolet light UVB. The cells were irradiated with UVB at 5 and 10 mJ/cm2. Total RNA and gDNA samples were collected 24 h after UVB irradiation, and protein samples were collected six hours after UVB irradiation. Samples were analyzed by (A,B,D) qRT-PCR, and (C) Western blot analysis. The nc886 mRNA level was normalized with 18S rRNA and the methylated-nc886 level was normalized with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The levels of expression were compared to the control for (A) nc886, illustrated in a fold change. A percentage compared to the control was used to indicate the levels for (B,D) methylation of nc886. # p < 0.01, compared to basal levels; $ p < 0.01, compared between (−) Aza-dC and (+) Aza-dC groups.