Figure 3.

Effect of Laminaria japonica extract (LJE) on the production of inflammation-related proteins increased by UVB. The cells were treated with LJE at a concentration of 100 µg/mL for 48 h prior to UVB irradiation (5 mJ/cm²). After 24 h of irradiation, RNA, gDNA samples, and cell culture fluid were collected. mRNA and gDNA samples were analyzed with qRT-PCR. A fold change was used to compare nc886 (A expression and (B) methylation, (C) MMP-9, and (D) COX-2 to the control. The measured values for (E) MMP-9, (F) PGE₂, (G) IL-8, and (H) TNF-α were indicated in an absolute concentration for each. * p < 0.05, # p < 0.01, compared to basal levels; ^o p < 0.05, ^$ p < 0.01, compared between (−) LJE and (+) LJE groups.