Figure 1.

Steps involved in the construction of the metabolic GRS. Fifteen SNPs were genotyped in our study; however, the GRS analysis was based only on 12 SNPs as 3 SNPs were not in the HWE. Three different GRSs, including the 12-SNP GRS, 8-SNP GRS and the 4-SNP GRS were constructed. In the 12-SNP GRS analysis, no significant results were identified, which could be because 4 of the SNPs had MAF of less than 5%. Therefore, the 4 SNPs were excluded, and an 8-SNP GRS was created. No significant findings were observed using the 8-SNP GRS; this could be because four of the eight SNPs have not shown a consistent association with obesity-related traits in other populations. Hence, these four SNPs were removed and a 4-SNP GRS including those SNPs that have shown consistent associations with obesity across several populations was constructed. Abbreviations: SNP: single nucleotide polymorphisms; GRS: genetic risk score; HWE: Hardy–Weinberg equilibrium; MAF: minor allele frequency.