Table 2.
Estimated extracellular flux.
| Metabolite | Parental | FHdim | Percent change (%) | ||
|---|---|---|---|---|---|
| Flux (nmol/106 cells/h) |
95% CI (LB, UB) |
Flux (nmol/106 cells/h) |
95% CI (LB, UB) |
||
| Uptake | |||||
| Glucose | 578.8 | (541.5, 616.1) | 533.5 | (499.3, 567.8) | − 8 |
| Glutamine | 82.7 | (77.3, 88.1) | 52.7 | (49.3, 56.1) | − 36 |
| Cysteine | 4.1 | (3.8, 4.5) | 3.3 | (3.1, 3.6) | − 19 |
| Serine | 22.0 | (20, 24.1) | 16.2 | (15.2, 17.3) | − 26 |
| Arginine | 8.7 | (8.1, 9.3) | 9.6 | (8.9, 10.4) | 10 |
| Secretion | |||||
| Lactate | 1,087.7 | (1,036.9, 1,138.5) | 1,009.8 | (975.1, 1,044.6) | − 7 |
| Pyruvate | 61.7 | (55.5, 67.8) | 57.8 | (52, 63.6) | − 6 |
| Alanine | 17.1 | (16.2, 18.1) | 19.4 | (18.4, 20.3) | 13 |
| Proline | 13.9 | (13.4, 14.3) | 1.5 | (1.4, 1.5) | − 89 |
| Ratio of lactate secretion flux to glucose uptake flux | 1.88 | 1.89 | |||
Percent change is defined as the ratio of the difference between parental and FHdim cells to the flux value in parental cells. Positive or negative value means increased or decreased flux in FHdim cells, respectively. CI, confidence interval; LB, lower bound; UB, upper bound.