Table 2.

Effect of VIP, VPAC1 agonist, and VPAC2 agonist on intracellular cAMP levels in PBMC. Intracellular cAMP levels were determined by ELISA in PBMC unstimulated or stimulated for 60 min with VIP (10 nM), VPAC1 agonist (10 nM), or VPAC2 agonist (10 nM). Determinations were done in duplicate.

	Healthy donors (n = 18)	Hyperthyroid GD (n = 9)	Euthyroid GD (n = 5)
Basal	0.38 ± 0.09	0.24 ± 0.05	0.31 ± 0.23
VIP, 10 nM	1.34 ± 0.22	0.42 ± 0.85*	0.51 ± 0.09
VPAC1 agonist, 10 nM	1.76 ± 0.25	0.47 ± 0.05**	0.05 ± 0.04***
VPAC2 agonist, 10 nM	0.97 ± 0.18	0.97 ± 0.23	1.6 ± 0.41

Data are expressed as pmol cAMP/mg protein, and means and SEM are shown. P values less than 0.05 were considered significant (*P < 0.05; **P < 0.001; ***P < 0.001), versus healthy donors. For more details, see “Methods” section.