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. 2020 Jul 21;9(7):429. doi: 10.3390/antibiotics9070429

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterisation of bioactive compounds in C. nutans extract. (A) The biological activities of four fractions—hexane fraction (F1), dichloromethane fraction (F2), ethyl acetate fraction (F3), and water fraction (F4), respectively—derived from sequential fractionation to reduce LPS-induced cell death were tested using Prestoblue™ reagent (* indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001). (B) The mass spectra and molecular structure of three overlapped majorcompounds—glyceryl 1,3-disterate (C₃₉H₇₆O₅), m/z 647.5589, exact mass 624.5681 (B1); kaempferol 3-O-feruloyl-sophoroside 7-O-glucoside (C₄₃H₄₈O₂₄), m/z 949.2620, exact mass 948.2526 (B2); and hydroxypthioceranic acid (C₄₆H₉₂O₃), m/z 710.7358, exact mass 692.7030 (B3). (C) TLC chromatogram of C. nutans extract (1); hexane fraction (2); dichloromethane fraction (3); ethyl acetate fraction (4); glyceryl 1,3-disterate, Rf 0.58 (4); and water fraction (5) using hexane/chloroform/ethyl acetate/methanol/water/formic acids 3:3:2:2:0.1:0.1, sprayed with 0.5% Anisaldehyde-H2SO4 reagent and heat at 120 °C for 10 min, recorded under visible light (a) and UV 366 nm (b).