Screening of antibacterial activity of herb extracted. In total, 14 herbs were extracted using ethanol/water at 7:3. Antibacterial activity of herb extracts against E. coli was tested at the equal concentrations of extracts (500 mg/mL) using the disc diffusion agar agar method. The clear zone was measured and summarized in the table (mean ± standard deviation) In total, 14 herbs were extracted using ethanol/water at 7:3. Antibacterial activity of herb extracts against E. coli was tested at the equal concentrations of extracts (500 mg/mL) using the disc diffusion agar agar method. The clear zone was measured and summarized in the table (mean ± standard deviation).