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. 2020 Aug 3;10:13016. doi: 10.1038/s41598-020-69495-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conditioned media from mouse cMSC suppress cardiomyocyte apoptosis after mouse myocardial infarction. (a) Schematic representation of cMSC-conditioned medium production, concentration and intramyocardial injection after LAD ligation. Concentrated conditioned or control media were injected into the infarct border zone, one site ~ 1 mm beneath the suture and a second site more apical. Images modified from Servier Medical Art website, a free medical image database with a licence under Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/). (b) Representative immunohistochemistry images of TUNEL staining, 24 h after myocardial infarction. An α-actinin antibody was used to demarcate cardiomyocyte identity. Scale bars 20 μm. See also Supplementary Fig. S11. (c) Bar graph of TUNEL staining in cardiomyocytes in the remote myocardium (intraventricular septum, IVS), infarct site, and border zone. Data are shown as the mean ± SEM. n = 3; *p < 0.05.