Figure 1.

High fat diet (HFD) administration leads to increased expression of Myo-inositol oxygenase (MIOX) and perturbations in the biomarkers of renal injury in different strains of mice. Panel (A): following HFD administration, an increase in the MIOX expression was observed in wild type (WT) mice and a much higher increase was seen in MIOX transgenic (MIOX-TG) mice. No increase in MIOX expression was observed in MIOX^KO mice fed with HFD. The ob/ob mice had very high MIOX expression and it was reduced to undetectable levels in ob/MIOX^KO mutant mice. Panel (B,C): a parallel increase in the levels of both the renal injury markers, i.e., Kidney Injury Molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) RNA, was observed in various strains of mice following HFD administration, and the most notable increase was observed in MIOX-TG mice. No increase in their expression was observed in MIOX^KO mice. The ob/ob mice had a very high expression of KIM-1 and NGAL and it was remarkably reduced in ob/MIOX^KO mice. (n = 6; * p < 0.05 compared with the control group, # p < 0.05 compared with the HFD group, one-way ANOVA with Dunn’s multiple comparisons).