Figure 2.

MIOX overexpression alters the expression of the metabolic sensor p-AMPKα in renal proximal tubules in a state of obesity. Panels (A–H): by immunofluorescence microscopy, a decrease in the expression p-AMPKα in the proximal tubular epithelia (arrowheads) was observed in WT, MIOX-TG and MIOX-KO mice following HFD administration. The lowest expression was observed in MIOX-TG mice, and MIOX-KO mice had discernible expression comparable to WT mice. The ob/ob mice had no expression of p-AMPKα, while it became detectable in some of the renal tubular epithelia in ob/MIOX^KO mice (magnification 200×). Panels (I,J): Western blot data revealed changes in the expression of p-AMPKα that were parallel to those observed by immunofluorescence microscopy in various strains of mice in a state of obesity.