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. 2020 Jul 3;9(7):536. doi: 10.3390/pathogens9070536

Figure 1.

Figure 1

Reduction of Chandler scrapie prion in ScN2a cell lysate after CAC-717 treatment. Cell lysates of N2a cells (N2a) or scrapie prion (Chandler)-infected N2a cells (ScN2a) in phosphate-buffered saline (PBS) were mixed with an equal volume of CAC-717 (+) or PBS alone (–) as a negative control. Samples were incubated at 25 °C for 1 h and then subjected to either treatment with proteinase K (PK) to determine PrPres levels (PK(+)) or no treatment to determine total PrP (PK(-)). Samples were analyzed by SDS-PAGE followed by Western blotting using anti-PrP antibody SAF83. PrPres was not detected in the ScN2a cell lysate treated with CAC-717, but total PrP levels were almost the same in CAC-717-treated and PBS-treated samples of both uninfected N2a and infected ScN2a cells. Molecular mass markers (kDa) are indicated on the right-hand side of the gel.