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Effects of LHMW on the mRNA expression of tyrosinase, Trp1, and Dct. α-MSH alone or simultaneously with LHMW was added to B16 cells and cultured for 24 h. The mRNA expression levels of tyrosinase, Trp1, and Dct were analyzed by real-time PCR and normalized to that of GAPDH. The average value of the control was expressed as 100% (mean ± SD, n = 6, **; p < 0.01, ***; p < 0.001 vs. control, ##; p < 0.01, ###; p < 0.001 vs. vehicle).
