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. 2020 Jul 3;12(7):1988. doi: 10.3390/nu12071988

Figure 3.

Figure 3

L-theanine reduced the expression matrix-degrading enzymes and pro-inflammatory mediates in IL-1β-stimulated rat chondrocytes. (A) Western bolt analysis of MMP-3 and MMP-13 in chondrocytes treated with L-theanine (50, 100, 200 μM) for 24 h. (B) Real time PCR analysis of gene expression of MMP-3 and MMP-13 in chondrocytes treated with L-theanine (50, 100, 200 μM) for 24 h. (C) Western bolt analysis of cyclooxygenase-2 (COX-2) and iNOS in chondrocytes treated with L-theanine (50, 100, 200 μM) for 24 h. (D) Enzyme-linked immunosorbent assay (ELISA) of prostaglandin E2 (PGE-2) and NO in the cell supernatant treated with L-theanine (50, 100, 200 μM) for 24 h. Values are the mean ± SD; ** p < 0.01, vs. IL-1β treatment group. ## p < 0.01 vs. L-theanine (100 μM) treatment group. C, control.