Table 1.
Sequences of primers and probes directed to E, N, and RdRp genes and used for the molecular detection of SARS-CoV-2.
| ID | Name | Sequences (5’-3’) | Reference | |
|---|---|---|---|---|
| 1 | RdRP_SARSr-F | GTGARATGGTCATGTGTGGCGG | [13] | |
| RdRp | RdRP_SARSr-R | CARATGTTAAASACACTATTAGCATA | ||
| RdRP_SARSr-P | FAM- CAGGTGGAACCTCATCAGGAGATGC-BHQ1 | |||
| 2 | ORF1ab-F (China) | CCCTGTGGGTTTTACACTTAA | [23] | |
| ORF1ab-R (China) | ACGATTGTGCATCAGCTGA | |||
| ORF1ab-Probe (China) | CCGTCTGCGGTATGTGGAAAGGTTATGG | |||
| 3 | HKU-ORF1b-nsp14F (Hong Kong) | TGGGGYTTTACRGGTAACCT | ||
| HKU-ORF1b-nsp14R Hong Kong | AACRCGCTTAACAAAGCACTC | |||
| HKU-ORF1b-nsp14P Hong Kong | TAGTTGTGATGCWATCATGACTAG | |||
| 4 | nCoV_IP2-12669Fw | ATGAGCTTAGTCCTGTTG | [24] | |
| nCoV_IP2-12759Rv | CTCCCTTTGTTGTGTTGT | |||
| nCoV_IP2-12696 Probe (+) | AGATGTCTTGTGCTGCCGGTA [5’]Hex [3’]BHQ-1 | |||
| 5 | nCoV_IP4-14059Fw | GGTAACTGGTATGATTTCG | ||
| nCoV_IP4-14146Rv | CTGGTCAAGGTTAATATAGG | |||
| nCoV_IP4-14084 Probe (+) | TCATACAAACCACGCCAGG [5’]Fam [3’]BHQ-1 | |||
| nCoV_2019 Forward | CAAATTCTATGGTGGTTGGCACA | [25] | ||
| 6 | nCoV_2019 Reverse | GGCATGGCTCTATCACATTTAGG | ||
| nCoV_2019 Probe | FAM- ATAATCCCAACCCATRAG-MGB | |||
| N-gene | 1 | N_Sarbeco_F | CACATTGGCACCCGCAATC | [13] |
| N_Sarbeco_R | GAGGAACGAGAAGAGGCTTG | |||
| N_Sarbeco_P | FAM- ACTTCCTCAAGGAACAACATTGCCA-BHQ1 | |||
| 2 | N-F (China) | GGGGAACTTCTCCTGCTAGAAT | [23] | |
| N-R (China) | CAGACATTTTGCTCTCAAGCTG | |||
| N-probe (China) | CAGACATTTTGCTCTCAAGCTG | |||
| 3 | HKU-NF (Hong Kong) | TAATCAGACAAGGAACTGATTA | ||
| HKU-NR (Hong Kong) | CGAAGGTGTGACTTCCATG | |||
| HKU-NP (Hong Kong) | GCAAATTGTGCAATTTGCGG | |||
| 4 | NIID_2019-nCOV_N_F2 (Japan) | AAATTTTGGGGACCAGGAAC | ||
| NIID_2019-nCOV_N_R2 (Japan) | TGGCAGCTGTGTAGGTCAAC | |||
| NIID_2019-nCOV_N_P2 (Japan) | ATGTCGCGCATTGGCATGGA | |||
| 5 | WH-NIC N-F Thailand | CGTTTGGTGGACCCTCAGAT | ||
| WH-NIC N-R Thailand | CCCCACTGCGTTCTCCATT | |||
| WH-NIC N-P Thailand | CAACTGGCAGTAACCA | |||
| 6 | CDC N1 Forward | GACCCCAAAATCAGCGAAAT | [26] | |
| CDC N1 Reverse | TCTGGTTACTGCCAGTTGAATCTG | |||
| CDC N1 Probe | FAM- ACCCCGCATTACGTTTGGTGGACC-BHQ1 | |||
| 7 | CDC N2 Forward | TTACAAACATTGGCCGCAAA | ||
| CDC N2 Reverse | GCGCGACATTCCGAAGAA | |||
| CDC N2 Probe | FAM- ACAATTTGCCCCCAGCGCTTCAG-BHQ1 | |||
| 8 | CDC N3 Forward | GGGAGCCTTGAATACACCAAAA | ||
| CDC N3 Reverse | TGTAGCACGATTGCAGCATTG | |||
| CDC N3 Probe | FAM- AYCACATTGGCACCCGCAATCCTG-BHQ1 | |||
| E-gene | E_Sarbeco_Forward | ACAGGTACGTTAATAGTTAATAGCGT | [13] | |
| E_Sarbeco_Reverse | ATATTGCAGCAGTACGCACACA | |||
| E_Sarbeco_Probe | FAM- ACACTAGCCATCCTTACTGCGCTTCG-BHQ1 | |||