Table IV.

Univariate and multivariate Cox regression analyses of clinicopathological parameters and tumoral immune parameters.

A, Univariate analysis
Variables	Unfavorable/favorable	HR	95% CI	P-value
Sex	Male/Female	0.863	0.343–2.176	0.755
Age, years	≤50/>50	0.747	0.255–2.187	0.594
Tumor size, cm	≤5/>5	3.643	1.587–8.363	0.002
Histological grade	I–II/III	4.624	1.722–12.421	0.002
Neural Invasion	No/Yes	2.168	0.969–4.847	0.060
Tumor thrombus	No/Yes	6.619	2.716–16.134	0.001
Lymphatic metastasis	No/Yes	11.528	2.702–49.184	0.001
pTN stage	I–II/III	18.732	2.525–138.976	0.004
TILs	High/Low	0.279	0.125–0.623	0.002
TLSs	High/Low	0.370	0.166–0.825	0.015
gcCD8+ TILs	High/Low	0.062	0.008–0.470	0.007
TILs-TLSs	High/Low	0.376	0.222–0.636	0.000
B, Multivariate Cox regression analysis
Variables	Unfavorable/favorable	HR	95% CI	P-value
Tumor size, cm	≤5/>5	0.896	0.198–4.046	0.886
Histological grade	I–II/III	1.009	0.277–3.671	0.990
Tumor thrombus	No/Yes	2.227	0.482–10.748	0.299
Lymphatic metastasis	No/Yes	1.251	0.135–11.560	0.844
pTN stage	I–II/III	5.594	0.336–93.237	0.230
TILs	High/Low	0.503	0.169–1.492	0.215
TLSs	High/Low	0.609	0.203–1.831	0.377
gcCD8+ TILs	High/Low	0.087	0.011–0.692	0.021
C, Multivariate Cox regression analysis
Variables	Unfavorable/favorable	HR	95% CI	P-value
Tumor size, cm	≤5/>5	1.208	0.273–5.355	0.803
Histological grade	I–II/III	0.806	0.208–3.116	0.754
Tumor thrombus	No/Yes	1.742	0.381–7.970	0.474
Lymphatic metastasis	No/Yes	0.980	0.103–9.296	0.986
pTN stage	I–II/III	7.159	0.419–122.410	0.174
gcCD8+ TILs	High/Low	0.067	0.008–0.561	0.013
TILs-TLSs	High/Low	0.247	0.069–0.882	0.031
D, Multivariate Cox regression analysis
Variables	Unfavorable/favorable	HR	95% CI	P-value
TILs	High/Low	0.322	0.124–0.835	0.020
TLSs	High/Low	0.396	0.148–1.056	0.064
gcCD8+ TILs	High/Low	0.059	0.008–0.451	0.006

Values in bold indicate P<0.05. TILs, tumor infiltrating lymphocytes; TLSs, tertiary lymphoid structures; HR, hazard ratio.