Fig. 1.

Inhibitory effect of STAT3 knockdown on cell proliferation and cap‐dependent translation. Cancer cells were treated with 5 nm of siCTRL (−) or siSTAT3 (+) for 72 h. (A) Equal amounts of extracts were analyzed by western blotting with the antibodies indicated. (B) Relative cell proliferation of each group measured by WST‐1 assay was compared with the siCTRL group of A549 (n = 4). (C) Cell cycle distribution was analyzed with FACS (n = 3). (D) Diagram of bicistronic luciferase reporter is shown (top). Luciferase activities were measured by a dual‐luciferase assay, and the Renilla/firefly luciferase luminescence ratio was calculated for cap‐dependent translational activity (n = 4). Data are presented as mean ± SEM. Statistically significant differences are marked with *P < 0.05, **P < 0.01, and ***P < 0.001, respectively (t‐test).