Fig. 4.

Correlation of STAT3 knockdown‐induced 4E‐BP1 dephosphorylation and downregulation of mTORC1. HCT116 cells were transfected with 5 nm of siSTAT3 or siCTRL for 72 h. Cell lysates were immunoprecipitated with mTOR antibody, followed by western blotting with the indicated antibodies using IP and input samples. Representative images of three independent experiments are shown.