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. 2020 Jun 29;14(8):1850–1867. doi: 10.1002/1878-0261.12735

Fig. 5.

Fig. 5

Intermediation of mLST8 in STAT3‐dependent 4E‐BP1 phosphorylation. (A) Western blotting (top) and mLST8 protein level were quantified at 72 h after 5 nm of siRNA treatment in human cancer cells. The relative intensity of mLST8 to GAPDH was normalized to that of siCTRL group of A549 (bottom; n = 3). Data are presented as mean ± SEM. Statistically significant differences are marked with *P < 0.05, **P < 0.01, and ***P < 0.001, respectively (t‐test). (B) HCT116 cells were transfected with 5 nm of siCTRL or siMLST8 for 24 h. Equal amounts of HCT116 cell lysates were immunoprecipitated with mTOR antibody. The proteins in the immunoprecipitated and input were analyzed with western blotting using the antibodies indicated. Representative images of three independent experiments are shown.