Fig. 3.

MSP activation of the RON receptor induces proliferation of KP and KB1P cells in vitro. (A) Proliferation of two KP and two KB1P cell lines in response to 100 ng·mL⁻¹ MSP, 1 µm BMS‐777607, or both reagents. For combined treatments, cells were pretreated with BMS‐777607 for 1 h before the addition of MSP. For all treatments, cells were re‐fed every 24 h with fresh MSP and BMS‐777607 as denoted. DNA content was measured at 72 h post‐treatment. (B) Proliferation of two KP and two KB1P cell lines transduced with control pLKO.1 or shRNA vectors against Mst1r mRNA following 48‐h treatment with recombinant MSP as above. For both A and B, data points shown are technical replicates from 1 representative experiment from three repeated experiments. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by one‐way ANOVA followed by Dunn’s post hoc test.