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. 2020 Jul 18;12(7):2138. doi: 10.3390/nu12072138

Figure 2.

Figure 2

The effect of prenylflavonoids (1 µM) on the normalized mRNA expression of GSTA1/2 (a), GSTP1 (b), SULT1A1 (c) and COMT (d) in proliferating CaCo-2 cells after 24 h and 72 h (n = 3). The normalized expression level was calculated using the 2−ΔΔCt method with the geometric mean of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-2 microglobulin (B2M) as a reference gene. Results are presented as the mean ± SD of three independent experiments. Statistical analyses were performed using a one-way ANOVA with Dunnett’s test: p < 0.05 (* or §), p < 0.01 (§§), p < 0.001 (§§§); * different from control after 24 h, $ different from control after 72 h.