Table 1.

Retention time (Rt), wavelengths of maximum absorption (λmax), mass spectral data, and the identification and quantification of phenolic compounds in fresh juice and purified juice of Viburnum opulus fruit.

Peak No.	Phenolic Compound	Rt (min)	λmax (nm)	[M − H]−/ [M + H]+ (m/z)	Fragment(s) Ions (m/z)	Content (mg/g)
						Fresh Juice (FJ)	Purified Juice (PJ)
1	Neochlorogenic acid 1	4,50	318	353	191, 179	0.007 ± 0.001	0,215 ± 0.019
2	Procyanidin dimer B1 1	5.12	276	577	407, 289	0.759 ± 0.003	47.596 ± 0.148
3	Caffeoylquinic acid derivative I 2	5.17	323	707	191	0.015 ± 0.000	1.289 ± 0.058
4	Caffeoylquinic acid derivative II 2	5.35	323	707	191	0.024 ± 0.002	1.051 ± 0.008
5	Procyanidin trimer I 3	5.60	278	865	577, 289, 243	0.112 ± 0.001	6.866 ± 0.342
6	(+)-Catechin 1	5.70	278	289	245, 202	0.657 ± 0.006	40.729 ± 0.596
7	Caffeoylquinic acid derivative III 2	5.74	323	707	191	0.017 ± 0.001	1.220 ± 0.020
8	Procyanidin trimer II 3	5.87	275	865	577, 289, 243	0.030 ± 0.006	2.634 ± 0.270
9	Chlorogenic acid 1	6.20	318	353	191, 707 a	8.039 ± 0.145	645.492 ± 1.984
10	Cryptochlorogenic acid 1	6.50	323	353	191, 173	0.004 ± 0.000	0.484 ± 0.023
11	Procyanidin dimer B2 1	6.61	278	577	407, 289	0.199 ± 0.002	11.540 ± 0.148
12	Gallocatechin gallate 4	6.84	280	457	169	0.031 ± 0.000	1.876 ± 0.085
13	Caffeoylquinic acid 4	7.31	313	353	191, 133, 707 a	0.745 ± 0.001	44.344 ± 0.176
14	(−)-Epicatechin 1	7.40	278	289	245, 202	0.135 ± 0.002	8.002 ± 0.116
15	Cyanidin-3-sambubioside 1	7.53	515	581+	287	0.093 ± 0.000	7.010 ± 0.003
16	Cyanidin-3-glucoside 1	7.74	515	449+	287	0.139 ± 0.000	13.583 ± 0.799
17	Procyanidin dimer C1 1	7.86	278	865	407, 289	0.033 ± 0.001	3.212 ± 0.351
18	B-typeprocyanidin dimer derivative I 5	7.96	276	739	577, 289	0.016 ± 0.000	2.071 ± 0.097
19	Cyanidin-3-rutinoside 1	8.10	516	595+	287	0.068 ± 0.001	5.072 ± 0.016
20	Procyanidin trimer III 3	8.32	278	865	577, 289, 243	0.032 ± 0.000	1.796 ± 0.053
21	Caffeoylquinic acid derivative IV 2	8.40	325	705	513	0.034 ± 0.000	3.306 ± 0.014
22	Caffeoylquinic acid derivative V 2	8.56	325	705	513	0.034 ± 0.000	3.268 ± 0.010
23	B-type procyanidin dimer derivative II 5	8.92	278	739	577, 289	0.035 ± 0.000	2.293 ± 0.094
24	Feruloylquinic acid I 3	9.43	325	367	193, 134	n.d.	5.722 ± 0.021
25	Quercetin-3-vicianoside 6	9.70	351	595	301, 300, 271	0.020 ± 0.000	1.266 ± 0.007
26	Procyanidin dimer 3	10.03	278	577	407, 289	0.024 ± 0.001	1.602 ± 0.258
27	Feruloylquinic acid II 3	10.13	304	367	193, 134	n.d.	0.528 ± 0.005
28	Quercetin-3-galactoside 4	10.18	365	463	300, 271	n.d.	0.149 ± 0.011
29	Quercetin-3-rutinoside 1	10.29	344	609	301, 270, 151	0.016 ± 0.000	0.921 ± 0.007
30	(Epi)catechin derivative I 2	11.13	280	451	289, 161	0.103 ± 0.001	6.998 ± 0.221
31	(Epi)catechin derivative II 2	11.27	280	451	289, 161	0.080 ± 0.001	6.006 ± 0.165
32	Quercetin-3-rhamnoside 4	11.78	344	447	301, 270, 255, 227	0.007 ± 0.000	0.491 ± 0.002

n.d.—not detected; ^a dimeric adduct. Results are expressed as a mean ± standard deviation (n = 3). The values expressed differ significantly (one-way ANOVA and Duncan’s test, p ≤ 0.05). 1—identification based on a comparison of retention time, UV-vis spectra (200–600 nm) and MS data for standards; 2,3,4,5—identification based on a comparison of molecular ions and typical ion fragments with published data; 2—[6], 3—[7], 4—[8], 5—[9], 6—[10].