Figure 2.

Plasma Hcy, folate, vitamin B12, and NO concentrations and brachial artery FMD measurements. Plasma from obese subjects (n = 40) and non-obese controls (n = 40) were analyzed for Hcy (A), folate (B), and vitamin B12 (C) using specific ELISA assays and for nitrates + nitrites (NO metabolites) using the Griess chemical reaction assay (D). Percentage of brachial artery FMD was calculated by subtracting the mean baseline diameter from the largest mean values obtained after cuff deflation in obese subjects (n = 40) and non-obese controls (n = 40) (E). All measurements are presented as means ± standard error (SE). * (p < 0.05) for comparing obese subjects with controls.