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. 2020 Jul 19;12(7):2146. doi: 10.3390/nu12072146

Figure 5.

Figure 5

Effects of Thymocid® on the expression of melanogenesis-related genes and proteins in melanoma B16F10 cells. Cells were treated with Thymocid® (20 µg/mL) for 24, 48, and 72 h and the expression of genes including MITF, TYR, TYRP1, and TYRP2 were determined by real-time qPCR assay (A). B16F10 cells were treated with Thymocid® (2.5 and 10 µg/mL) for 72 h, and the expression of genes including MITF, TYR, TYRP1, and TYRP2 were determined by real-time qPCR (B). B2M was used as an internal control in real-time qPCR assay. Significance was defined as * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001 when compared to the control group. Values are presented as the means ± S.D. from three experimental replicates.