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. 2020 Jul 9;12(7):2040. doi: 10.3390/nu12072040

Figure 2.

Figure 2

Effect of Nem, Cp5 and Cp17 on M2-like macrophages. (A–C) MTT assay: M2-like macrophages were exposed to increasing concentrations of Nem (A), Cp5 (B) and Cp17 (C) for 24, 48 and 72 h; (D) mRNA levels of IL8, IL10, CCL2 and VEGF after 24 h of exposure to 21 µM, 44 µg/mL, 16 µg/mL of Nem, Cp5 and, Cp17, respectively (IC50/72 h); (E) MMP-9 activity was evaluated by zymography in different supernatants (THP-1-CM, M2-CM, Nem-CM, Cp5-CM and Cp17-CM); (F) Cell cycle analysis after 24 h of exposure to 21 µM, 44 µg/mL, 16 µg/mL of Nem, Cp5 and, Cp17, respectively (IC50/72 h). Data are reported as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.001, *** p < 0.0001, **** p < 0.0001 vs. MΦ or THP-1-CM, & p < 0.05, && p < 0.01, &&& p < 0.001, &&&& p < 0.0001 vs. M2-like macrophages or M2-CM, # p < 0.05 vs. Nem-CM. N.D. Not detected. A.U. arbitrary units.